PTI’s time tested RatioMaster™, offering researchers solid, dependable, sensitive detection for the collection and analysis of ratio-metric photometry data for calcium, pH, and intracellular ion imaging!
The RatioMaster™ is a microscope-based ratio spectrofluorometer capable of dynamic ratio fluorescence measurements on a millisecond timescale. A xenon arc lamp provides high intensity, continuous broadband illumination. Alternating excitation wavelengths are selected by a computer-controlled high-speed random access monochromator coupled to a (user specified/supplied) inverted fluorescence microscope with a liquid light guide. Emitted light is collected from the sample and passed through a photometer having a bilateral translatable iris and a viewing eyepiece to a switchable analog/photon counting photomultiplier detector. Analog detection is used when emitted light is relatively intense; low light levels are detected by photon counting. All system functions are under computer control. Data is collected and analyzed by proprietary Windows based advanced fluorescence software.